dCas9-KRAB mRNA (unmodified)
dCas9-KRAB mRNA encodes the dCas9-KRAB fusion protein, a tool for gene silencing and transcriptional repression. The dCas9 (dead Cas9) component targets DNA precisely without cutting, while the KRAB (Krüppel-associated box) domain recruits complexes to inhibit gene expression. Delivered as mRNA, dCas9-KRAB ensures rapid, high transient expression without nuclear uptake, making it ideal for research in gene function, genetic diseases, and therapeutic development.
Croyez’s dCas9-KRAB mRNA is generated through in vitro transcription, with a Cap1-modified 5' end and a poly(A) tail at the 3' end to mimic mature mRNAs. These optimizations enhance stability and performance, ensuring the mRNAs function like natural mature mRNAs in cells.
Croyez’s dCas9-KRAB mRNA is generated through in vitro transcription, with a Cap1-modified 5' end and a poly(A) tail at the 3' end to mimic mature mRNAs. These optimizations enhance stability and performance, ensuring the mRNAs function like natural mature mRNAs in cells.
mRNA Length:
4982 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Gene function research, genetic disease studies, therapeutic development, genetic modification
Shipping Conditions:
Dry ice
4982 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Gene function research, genetic disease studies, therapeutic development, genetic modification
Shipping Conditions:
Dry ice
